Green Synthesis of Silver Nanoparticles and Testing its Effectiveness in Inhibiting E. coli, Staphylococcus Aureus Isolated from Urinary Tract Infections of Pregnant Women

Abstract

The aim of the study was to identify the E. coli and S. aureus bacteria isolated from pregnant women with urinary tract infections that silver nanoparticles derived from green leaves inhibited. The silver nanoparticles showed inhibitory activity against these two types by Well diffusion method. towards bacteria. The result of the inhibition diameters by Well diffusion method were (35,33,30,25) for E.coli and (37,34,28,23) for S.aureus bacteria at concentrations (10,7.5,5,2.5)% respectively through the absence of colony growth on the surface of Muller Hunton agar plates at those concentrations. The use of nanoparticles as a result of their effective properties in inhibiting bacteria, provided that there are no side effects that affect its work. To make silver nanoparticles in an eco-friendly manner—that is, without heating the plant extract and solution or utilizing chemical catalysts during the reaction—one milliliter of strawberry leaf extract and fifty milliliters of silver nitrate solution—prepared at a concentration of one millimeter at room temperature for one to eight hours—were combined. Using UV spectrophotometers, scanning electron microscopy (SEM), XRD, and FTIR, it was determined that the preparation had begun when the mixture's color intensity increased after each of these eight hours had gone. The absorbance at 378 nm was visible in the UV-VIS spectrum. According to the results of scanning electron microscopy, the produced silver nanoparticles ranged in size from 37.35 nm. The biological method of producing silver nanoparticles in this low-cost and eco-friendly manner has shown promising results in preventing the growth of bacteria isolated from wound infections.