Effectiveness of Mixed Gycyrrhiza Glabra and Punica Granatum Plant Extract and Honey against Helicobacter Pylori Bacteria

Abstract

Objectives: The current study intends to determine the effectiveness of honey and the plants alcoholic extracted Gycyrrhiza glabra, Punica granatum, and honey alone or in combination to determine the antibacterial action of these plants against Helicobacter pylori. Methods: For made the cured extraction of plants G. glabra and P. granatum, ethanol is the solvent used. The most effective concentration of solvent optimization was found by extracting and diluting honey, testing its efficiency against the H. pylori bacteria, and determining the “minimum inhibitory concentration” (MIC) of the additional effective extract.

Results: The extraction in vitro demonstrated effect against H. pylori in contrast to the antibiotic. Results observed in pathogenic bacteria by inhibition zone diameter the mixed plant extract of G. glabra and P. granatum with honey in concentrations (50, 25, 12.5, mg/mL) (26.20, 14.86, 8.13 mm) in arrangement, and all of this means that there is a significant variation in the P-value and little effectiveness when treated with plant extract of G. glabra and P. granatum (50, 25, mg/mL) (12.10, 5.46 mm) and with effectiveness in the mixed plant extract of G. glabra and L. usitatissimium in (50 mg/mL) (7.33 mm) without any significant variation in P-value and no effectiveness in the plant extract of G. glabra alone. Conclusion: Current studies will aid in the isolation of novel goods and medications. The findings of this study have demonstrated the presence of antibacterial and antioxidant components in three plant extracts and honey.